Table 2.
Information on the primary antibodies
| Detection | Clone | Host species | Dilution | Source | Goal |
|---|---|---|---|---|---|
| Ataxin-3 | – | Rabbit polyclonal | 1:750 | Henry Paulson laboratory | Aggregate marker |
| Ataxin-3, epitope E214-L233 | 1H9 | Mouse monoclonal | 1:2,500 | Chemicon (Millipore), Billerica, MA, USA | Aggregate marker |
| Ubiquitin | p4d1 | Mouse monoclonal | 1:750 | Cell Signaling Technology, Danvers, MA, USA | Aggregate marker/marker for proteasomal activity |
| P62 | Sc-25575 | Rabbit polyclonal | 1:100 | Santa Cruz, Delaware, CA, USA | Aggregate marker/marker for proteasomal activity |
| Polyglutamine stretch | 1C2 | Mouse monoclonal | 1:2,000 | Chemicon (Millipore), Billerica, MA, USA | Aggregate marker |
| Alpha-synuclein | – | Sheep polyclonal | 1:2,000 | W.P Gai laboratory | Aggregate marker |
| Hyperphosphorylated tau | AT8 | Mouse monoclonal | 1:2,000 | Innogenics, Ghent, Belgium | Aggregate marker |
| Microtubule-associated protein tau | AT270 | Mouse monoclonal | 1:3,000 | Pierce Endogen, Rockford, IL, USA | Axonal marker |
| Neurofilament | ab 30309 | Rabbit polyclonal | 1:100 | Abcam, Cambridge, UK | Axonal marker |
| Tryptophan hydroxylase | PH8 | Mouse monoclonal | 1:1,000 | Chemicon (Millipore), Billerica, MA, USA | Marker for serotonergic cells |
| Tyrosine hydroxylase | MAB152 | Rabbit polyclonal | 1:2,000 | Chemicon (Millipore), Billerica, MA, USA | Marker for dopaminergic and noradrenergic cells |
| TDP-43 | – | Rabbit polyclonal | 1:1,000 | Georg Auburger laboratory | Aggregate marker |
Antigen detected by antibody, clone or catalog designation (omitted with donated antibodies), host species and clone type, working dilution for the antibody, source of the antibody, specific goal of the antibody usage