Table 3.
Summary of the main laminar staining patterns with antibody panel in control tissues
| Layer | Paediatric control (no epilepsy) | Adult control (no epilepsy) | Epilepsy (no MCD) (where different from non-epilepsy controls) |
|---|---|---|---|
| I | Tbr2 (Ma) | ER81 (A) | Otx1 (I) |
| ER81 (A) | Cux 1 (N) | Tbr2 (M) | |
| Cux 1 (I, M) | Pax6 (I) | ||
| MASH1 (P) | |||
| II | Otx 1 (I) | Tbr1 (A) | Otx 1 (I, S) |
| Cux 1 (N) | Map1b (P) | Tbr2 ± (I, M) | |
| ER81 (A) | N200 ± (P) | ||
| ER81 (A) | |||
| Cux1 (N) | |||
| III | ER81 (A) | Tbr1 (P) | Otx 1 (I, S) |
| Map1b (P) | Map1b (P) | ||
| N200 ± (P) | |||
| ER81 (A) | |||
| Cux1 (N) | |||
| IV | Map1b ± (N) | Tbr1 (N) | Otx 1 (I, S) |
| Otx1 (I) | ER81 (A) | ||
| Cux 1 (N) | Cux1 (N) | ||
| ER81 (A) | |||
| V | Tbr1 ± (P) | Tbr1 (P) | Otx 1 (I, S) |
| Map1b ± (P) | Map1b (P) | ||
| N200 ± (P) | N200 (P) | ||
| ER81 (A) | ER81 (A) | ||
| MASH1 ± (P) | Cux1 (N) | ||
| VI | Tbr1 (P) | Tbr1 (P) | Otx 1 (I, S) |
| Map1b ± (P) | Map1b (P) | ||
| N200 ± (P) | N200 (P) | ||
| ER81 (A) | ER81 (A, S) | ||
| Otx 1 (I) | Cux1 (N) | ||
| MASH1 ± (P) | |||
| White matter | Tbr1 (I) | Tbr1 (A) | Otx 1 (I, S) |
| Tbr2 (Ma) | N200 (P) | ||
| Otx1 (I) | Map1b ± (A) | ||
| ER81 (A) | ER81 (A) | ||
| Cux 1 (I) | Pax6 (I) | ||
| MASH1 ± (P) | Cux 1 (N) | ||
| Pax6 (I) | MASH1 ± (P) |
For the epilepsy control group markers are listed only where they deviated from other control groups. Markers underlined indicate more intense and frequent labelling of cells in the corresponding lamina
MCD malformation of cortical development, ± indicates less frequent cells were seen to label, P pyramidal cell, N non-pyramidal cell, A all types of mature neuronal cells, i.e. pyramidal and non-pyramidal, M small, multipolar cell with staining of cytoplasmic processes, including bipolar cells, I immature small cell of indeterminate lineage (small nucleus with little cytoplasm), S perineuronal satellite cells
aCo-expression with GFAP shown in controls in some populations