TABLE 2.
DEAB treatment in zebrafish, medaka, and Mexican tetra at different time points
| Species | DEAB treatment | Tooth induction |
|---|---|---|
| D. rerio | 5–11 hpf (5 μm) | − |
| D. rerio | 12–24 hpf (10 μm) | + |
| D. rerio | 18–30 hpf (10 μm) | − |
| D. rerio | 23–30 hpf (10 μm) | − |
| D. rerio | 20–28 hpf (10 μm) | − |
| D. rerio | 28–32 hpf (10 μm) | + |
| D. rerio | 40–56 hpf (10 μm) | − |
| O. latipes | Stage 20–stage 32 (30 μm) | − |
| O. latipes | 4–8 dpf (30 μm) | + |
| O. latipes | 3–7 dpf (60 μm) | + |
| O. latipes | 5–8 dpf (60 μm) | + |
| O. latipes | 4–7 dpf (100 μm) | + |
| O. latipes | 4–8 dpf (120 μm) | + |
| O. latipes | 4–8 dpf (160 μm) | + |
| A. mexicanus | 18–75 hpf (20 μm) | − |
| A. mexicanus | 24–75 hpf (20 μm) | − |
| A. mexicanus | 27–75 hpf (50 μm) | +/− |
| A. mexicanus | 30–75 hpf (50 μm) | + |
| A. mexicanus | 30–75 hpf (75 μm) | + |
| A. mexicanus | 40–75 hpf (50 μm) | + |
The presence of teeth was monitored by in situ hybridization with dlx2a and or dlx2b for the zebrafish and the Mexican tetra and by Alizarin red staining and dlx3b for the medaka.