Figure 1.

Aldehyde and CS lower SIRT1 levels in human lung epithelial cells. A) BEAS-2B cells were treated with varying concentrations of CSE (0.1–1.5%) or H₂O₂ (150, 250, or 500 μM) for 6 or 24 h. Cells were also treated for 1 or 6 h with either KO₂ (1 μM) or dry DMSO vehicle (Veh). Ctrl, untreated control. B) BEAS-2B cells were treated with acrolein (10 or 30 μM) for 6 h. C) SAECs were treated with CSE (0.5 and 1%) for 4 h. Whole-cell extracts from treated cells were used for immunoblot analysis for SIRT1. β-Actin was used as a housekeeping loading control. Blots are representative of ≥3 separate experiments (n=3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. controls.