Figure 10.

Altering cell thiol status affects post-translational modifications on cysteine residues of SIRT1 in response to aldehydes and oxidants. SIRT1 was immunoprecipitated (IP) from whole-cell extracts of BEAS-2B cells treated for 6 h with acrolein (30 μM), H₂O₂ (150 μM), and CSE (1%) in the presence or absence of a 2-h pretreatment with NAC (2 mM) (A) or wild type C57BL/6J mice exposed to air or CS (B). Free cysteine residues were labeled using maleimide-PEO₂-biotin and visualized using immunoblot with streptavidin conjugated to horseradish peroxidase. Blot is representative of three separate experiments (n=3). **P < 0.01, ***P < 0.001 vs. controls; ⁺P < 0.05 vs. CSE (1%).