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MALDI-TOF/TOF MS spectra of SIRT1 modified by reactive aldehydes. A) Recombinant human SIRT1 (30 μg) was modified with 4-HNE (30 μM) for 18 h at 37°C and analyzed by MALDI TOF/TOF mass spectrometry (AutoflexIII TOF/TOF MALDI mass spectrometer; Bruker Daltonics). Data were analyzed using Mascot 2.1.04. B) Spectra of the sequence (residues 467–492) containing a cysteine residue at 482 modified by 4-HNE; modified cysteine is denoted by bold and underscored font.
