Figure 2.

Oxidants, aldehyde, and CSE dose and time dependently decrease SIRT1 deacetylase activity. A, B) BEAS-2B cells were treated with varying concentrations of CSE (0.1, 0.5, 1, 1.5%), H₂O₂ (150 μM), and acrolein (30 μM) for 6 h (A) or 24 h (B). Cells were also treated with sirtinol (10 μM) and resveratrol (5 μM), a known inhibitor and activator of SIRT1, respectively. SIRT1 was immunoprecipitated from whole-cell extracts and used for the SIRT1 deacetylase activity assay. C) Cell extracts were also used for immunoblot for acetylated p53 (Lys 382) and p53 as a second measure of SIRT1 activity. Blots are representative of ≥3 separate experiments (n=3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. controls.