Figure 8.

Oxidative stress and CSE induce carbonylation on SIRT1. SIRT1 was immunoprecipitated (IP) from whole-cell extracts of BEAS-2B cells treated for 1 h with acrolein (30 μM), H₂O₂ (150 μM), and CSE (1%) in the presence or absence of a 2-h pretreatment with NAC (2 mM). Equal amount (100 μg) of immunoprecipitated SIRT1 protein was used for immunoblotting (IB). Carbonylation was detected by first derivitizing the samples with DNPH and immunoblotting with an anti-DNP antibody. Blot is representative of ≥3 separate experiments **P < 0.01, ***P < 0.001 vs. control; ⁺⁺P < 0.01 vs. CSE.