TABLE 1.
Peptides identified by MALDI TOF/TOF-MS from SIRT1 modified By 4-HNE
| Mass (Da) | Sequence position | Sequence | Peak |
|---|---|---|---|
| 1144.817 | 385–394 | GDIFNQVVPR | A |
| 1200.815 | 66–77 | GCPGAAAAALWR 2:Carbamidom ethyl (C) | B |
| 1547.042 | 637–649 | LDGNQYLFLPPNR | C |
| 1703.201 | 636–649 | RLDGNQYLFLPPNR | D |
| 1961.387 | 256–274 | IIVLTGAGVSVSCGIPDFR 13:Carbamidom ethyl (C) | E |
| 1963.323 | 342–358 | NYTQNIDTLEQVAGIQR | F |
| 2073.405 | 182–199 | IGPYTFVQQHLMIGTDPR | G |
| 2089.480 | 255–274 | KIIVLTGAGVSVSCGGIPDFR 14:Carbamidom ethyl (C) | H |
| 2448.817 | 445–466 | VRPVALIPSSIPHEVPQILINR | I |
| 2499.581 | 283–303 | LAVDFPDLPDPQAMFDIEYFR | J |
| 2653.705 | 520–543 | ELAYLSELPPTPLHVSEDSSSPER | K |
| 3239.535 | 467–492 | EPLPHLHFDVELLGDCDVIINELCHR 4-HNE (C), Carbamidom ethyl (C), on either residue 16 or 24 | L |