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Expression of PAX6, SIX3, SOX2, αA- and αB-crystallins during initial differentiation (d 0 to 14) of human ES cells into lens progenitor-like cells. A) qRT-PCR analysis of lens-lineage specific transcription factors PAX6, SIX3, and SOX2. B) qRT-PCR analysis of “early” αA- and αB-crystallin genes. C) Immunofluorescence detection of αA- and αB-crystallin proteins at d 12.
