Figure 3.

Longitudinal stretch phosphorylates Akt, IKK, IκBα, p50, and p65 in the mouse diaphragm. Diaphragms were stretched for 15 min either longitudinally or transversely to the direction of the muscle fibers. Cytoplasmic and nuclear lysates prepared from each diaphragm were resolved, 85 μg for each sample, by SDS-PAGE gel and immunoblotted with ether normal or phospho-specific Akt (A), IKK and IκBα (B), p50 and p65 (C), or tubulin (loading control) antibody. Gel pictures are representative of 3 independent experiments (n=3).