Figure 6.

Gene expression pattern searches revealed that C2orf40 was involved in skeletal development. A) The C2orf40 expression profile as obtained from our expression database. B) Approximately 500 genes clustered close to C2orf40 (left panel), revealing the existence of different expression patterns. The cluster of genes with the most similar expression pattern as that of C2orf40, indicated by the purple bar on the right, was expanded (right panel, with the location of C2orf40 indicated). Some significant GO categories associated with these clusters are shown on the right; they are all associated with skeletal development. C) In situ hybridization showed that C2orf40 was up-regulated in the vertebrae by wk 7–9. Cells with brown/yellow stains were considered positive. Note that C2orf40 had little expression in the limbs, different from the expression of C1orf61.