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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: Langmuir. 2003 Mar 4;19(5):1551–1556. doi: 10.1021/la026417v

Figure 1.

Figure 1

Schematic of the fabrication process for the neuron chamber. The top piece of the chamber is fabricated in PDMS by molding against a master that has a two-level photoresist pattern. Steps A and B show the formation of microgrooves (3 μm high and 10 μm wide) in the master using a thin photoresist layer. The size of the grooves was designed to limit the neurons in the somal chamber while allowing the growing neuritic processes to cross from one chamber to another. Steps C and D show the fabrication step for main compartments for soma and neurite chambers. Two chambers, separated by a barrier, form fluidically isolated areas that each hold less than 2 μL of fluid (100 μm high, 1500 μm wide, and 8 mm long). The top part of the device is formed by replica molding PDMS against the master (step E). Releasing the PDMS and sealing it to a flat substrate completes the neuron chamber fabrication (step F).