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Control of NP’s physicochemical properties. (a) Control of surface charge and lipid coverage of the hybrid NPs is elucidated by changes in zeta potential of the NPs in PBS using DSPE-PEG with modified functional groups of carboxyl, methyl, and amine (b) Control of NP size by varying PLGA viscosity and concentration in the organic stream. Flow ratios of aqueous to organic streams and rate were kept constant at 10 : 1 at a total flow rate of 55 µL/min.
