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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Arthritis Rheum. 2010 May;62(5):1383–1392. doi: 10.1002/art.27369

Figure 5. SirT1 levels are reduced in OA cartilage where PTP1B levels are elevated.

Figure 5

Normal and OA human cartilage samples were processed for immunohistochemistry for either SirT1 (A), PTP1B (B) or MMP13 (C) (left panels). The percent positive stained cells per field was determined and is shown in the graphs (right panels). Stained cells are reflective of greater than 10-fold intensity above the background (as determined by scanning densitometry). An average of 10 fields from three sections of six separate OA and Normal cartilage samples were assessed. Each field was blindly read by two different individuals. The images in A, B and C represent the intermediate layer of cartilage. B. Immunoblot of extracts from freshly isolated chondrocyte extracts from two normal and two OA cartilage samples probed with the indicated antibodies. Percent apoptotic cells in the OA and Normal cartilage samples were assessed by TUNEL assay (B). The error bars in all graphs indicate the standard deviation and the (*) indicates a statistically significant difference (p<0.05).