Table I.
Gene specific primers for quantitative RT-PCR.
| Gene | Forward primer (5'-to-3') | Reverse primer (5'-to-3') |
|---|---|---|
| ARG-1 | GTTTCTCAAGCAGACCAGCC | GCTCAAGTGCAGCAAAGAGA |
| B7H4 | CCCTGAAATACCAAAGCCAA | AGCTCCACTCAGCCAGTACC |
| CD33 | TTCCTCCTGTGGGTCTTCAC | CTTTCCAGGAGATGGCTCAG |
| C/EBPβ | AGCTGCTCCACCTTCTTCTG | AGCGACGAGTACAAGATCCG |
| c-kit L | TCCTGCAGATCCCTTCAGTT | ACACCACTGTTTGTGCTGGA |
| COX2 | GTTTTGACATGGGTGGGAAC | CCCTCAGACAGCAAAGCCTA |
| FLT3L | GGCCTCTAGCCAACTTCCTC | CAGTCCCCAGGACCTGCT |
| GAPDH | TTAAAAGCAGCCCTGGTGAC | CTCTGCTCCTCCTGTTCGAC |
| GM-CSF | GTCTCACTCCTGGACTGGCT | ACTACAAGCAGCACTGCCCT |
| IDO | TCAGGCAGATGTTTAGCAATG | GGCACACGCTATGGAAAACT |
| IL-10 | TCAAACTCACTCATGGCTTTGT | GCTGTCATCGATTTCTTCCC |
| IL-1β | GGAGATTCGTAGCTGGATGC | GAGCTCGCCAGTGAAATGAT |
| IL-4 | AGCGAGTGTCCTTCTCATGG | CAGCCTCACAGAGCAGAAGA |
| IL-6 | CTGCAGCCACTGGTTCTGT | CCAGAGCTGTGCAGATGAGT |
| iNOS | ATTCTGCTGCTTGCTGAGGT | TTCAAGACCAAATTCCACCAG |
| MCSF | CCAGCAACTGGAGAGGTGTC | GCGCTTCAGAGATAACACCC |
| NOX2 (NCF1) | CCTGCCTCAATAGGGAACATT | TCGTACCCAGCCAGCACTAT |
| PDL1 | TATGGTGGTGCCGACTACAA | TGCTTGTCCAGATGACTTCG |
| PDL2 | TGACTTCAAATATGCCTTGTTAGTG | GAAGAGTTCTTAGTGTGGTTATATG |
| TGFβ | GCAGAAGTTGGCATGGTAGC | CCCTGGACACCAACTATTGC |
| TNFα | AGATGATCTGACTGCCTGGG | CAGCCTCTTCTCCTTCCTGA |
| VEGF | CACACAGGATGGCTTGAAGA | AGGGCAGAATCATCACGAAG |