Table 3.
Crossing experiments
| Cross | Cross type | Female | Species/hybrid | Male | Species/hybrid | N of available tadpoles (at day 7 after spawning) | Ca.% Estimated hatching success |
N of tadpoles (2 months after spawning) | % Survival (2 months after spawning) | Remarks | Survival at day 40 after metamorphosis |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | F1 | Si41 | balearicus | Si11 | siculus | 200 | > 80 | 158 | 79 | malformations, some dwarfed or leucistic larvae | 150 |
| 2 | F1 | Si337 | balearicus | Si334 | siculus | 100 | > 80 | 98 | 98 | died through technical accident | N.A. |
| 3 | F1 | Si335 | siculus | Si336 | balearicus | 100 | > 80 | 93 | 93 | - | 4 |
| 4 | Backcross (F1 × parental species) | Si337 | balearicus | F1 Cross 13 | F1 (bal. × sic.) | 100 | > 80 | 84 | 84 | 67 (big), 17 (small) | 2 |
| 5 | Backcross (F1 × parental species) | Si335 | siculus | F1 Cross 13 | F1 (bal. × sic.) | 100 | > 80 | 91 | 91 | - | 0 |
| 6 | Backcross (F1 × parental species) | F1 Cross 11 | F1 (bal. × sic.) | Si334 | siculus | 100 | ca. 50 | 1 | 1 | - | 0 |
| 7 | Backcross (F1 × parental species) | F1 Cross 11 | F1 (bal. × sic.) | Si336 | balearicus | 28 | ca. 30 | 24 | 85.7 | 1 color mutant, all dead (at day 116 after spawning) | 0 |
| 8 | F2 (F1 × F1) | F1 Cross 11 | F1 (bal. × sic.) | F1 Cross 13 | F1 (bal. × sic.) | 100 | ca. 20 | 0 | 0 | all dead (at day 17 after spawning) | 0 |
| Control1 | Intra-specific mating | Ky109 | turanensis | Ky103 | turanensis | 50* | > 80 | 48 | 94 | - | 47 |
| Control2 | Intra-specific mating | Ky100 | pewzowi | Ky87 | pewzowi | 50* | > 80 | 47 | 90 | - | 45 |
Cross number, cross type, sexes, species, estimated hatching success, numbers of tadpoles at 7 day and two months after spawning, percentage of survival two months after spawning, remarks and survival at day 40 after metamorphosis for each cross. *Note, in controls from two related green toad species, only 50 tadpoles were further raised.