Table 2.
Characteristics of the mapped SSR loci from a Musa genomic library (mMaCIR102 to mMaCIR 305) and from EST sequences (mMECIR0494 to mMECIR0500).
| SSR | EMBL | motif | LG | forward primer | reverse primer | Ann (°C) | Size (bp) |
|---|---|---|---|---|---|---|---|
| mMaCIR102 | AM950328 | (AG)10,(TG)5 | 6 | TGTTGGATTGGCTTCATC | CTTCGTTCAATGGTCTCCT | 55 | 220 |
| mMaCIR103 | AM950329 | (CT)14, | 3 | CCTCTTCTCCCTGTGTTG | CGGTTTAACATACCTATTCTTG | 54 | 179 |
| mMaCIR105 | AM950331 | (CA)8,(CT)15 | 6 | CATCCACTTGCTTTTCCA | CTTCACGGCTTCCACA | 56 | 264 |
| mMaCIR108 | AM950334 | (CA)7,(CA)4, | 2 | ACGCATGGTAAAGTGGAA | ACATTCAAATCACGTTGCT | 55 | 111 |
| mMaCIR109 | AM950335 | (CA)13, | 4 | ACTCTAGTTCCAGAATAACTCCA | CAATCTTCATTAGCCAGTTGT | 55 | 204 |
| mMaCIR110 | AM950336 | (AC)7,(GA)6, | 3 | GGTGAACTGATGTGCGA | TCTTTCAACGGAATAAGCA | 55 | 244 |
| mMaCIR111 | AM950337 | (CA)8, | 6 | TCGTATGGAACAACAGTCC | CTTTCACCTTCAAACAGCA | 55 | 137 |
| mMaCIR112 | AM950338 | (CA)5,(CA)15 | 7 | GTTCGGCTGGAGGTAGTT | AAGAACACGAAGGCAGG | 55 | 330 |
| mMaCIR113 | AM950339 | (CA)10, | 10 | TCAAGTATTTCACCGTATTGC | TTACCACCCTGTCATCTTTC | 55 | 207 |
| mMaCIR114 | AM950340 | (AC)7,(CT)28 | 8 | GCAAGCCAAAGGGAA | ACCAACAAAGAATGGTGTAA | 54 | 222 |
| mMaCIR115 | AM950341 | (CA)2, | 11 | CAAGAGACTACCACCGAAGA | TGATTCTCACGACGTATGG | 55 | 114 |
| mMaCIR116 | AM950342 | (TC)2,(TC)20 | 11 | ACACAAAGAAACCAGCCA | CGTCCCATCGTCTCCT | 55 | 202 |
| mMaCIR117 | AM950343 | (TC)20, | 7 | GTTTGTGGAATAAGTGGGAA | ATGAGGGAGTTAGTGGTGG | 55 | 214 |
| mMaCIR119 | AM950345 | (CA)9,(TA)6,(CA)5, | 10 | TGAAAAGCAATCCAACCT | ACCCTGAAATGTTTGTCTTT | 54 | 395 |
| mMaCIR122 | AM950348 | (GT)8, | 7 | CGGTGACACTGGAAGGT | CAACTGAAGAACTGCCACTAA | 56 | 204 |
| mMaCIR124 | AM950350 | (AC)7, | 9 | ACCTTGACAGCCCTCTTC | ATCAATCATTTCTGGGGTT | 55 | 63 |
| mMaCIR129 | AM950355 | (CA)6, | 5 | CTAACCTTTGATTCTGTTTG | GTCCCTGATACACCATTC | 50 | 214 |
| mMaCIR130 | AM950356 | (TG)17, | 6 | TTAAACGTCTCCGTGTCTTC | TTGCATGAGGCTGGG | 56 | 311 |
| mMaCIR137 | AM950363 | (TC)12, | 4 | CGTATTCTACATCTGCTTCTTT | GCAGTGATTAGGTGATGATTT | 54 | 223 |
| mMaCIR138 | AM950364 | (CA)7, | 3 | TCATTCTCATGCGGAACT | CGGTGGATGTTGTTGG | 55 | 173 |
| mMaCIR139 | AM950365 | (GA)18, | 5 | TCGTCCCCTACTGCCT | ATGCTTCCGTTTGGCT | 55 | 187 |
| mMaCIR150 | AM950440 | (CA)10 | 3 | ATGCTGTCATTGCCTTGT | GAATGCTGATACCTCTTTGG | 55 | 238 |
| mMaCIR151 | AM950441 | (CT)21 | 3 | TATCCACCTCCTGGCAC | GCCAAACATCACCCAAC | 55 | 172 |
| mMaCIR152 | AM950442 | (CTT)18,(CT)17,(CA)6 | 4 | CCACCTTTGAGTTCTCTCC | TTTCCCTCTTCGATTCTGT | 55 | 163 |
| mMaCIR154 | AM950444 | (CT)17 | 4 | CATTCAGCATGGAAACCT | CTTCCTCAAACTGCTCCTC | 55 | 311 |
| mMaCIR156 | AM950446 | (TG)23 | 4 | CTTTCTGAAGGAAATTCTGAC | AGTGCAGCCCAATGAA | 54 | 210 |
| mMaCIR157 | AM950447 | (CA)9,(TA)7 | 11 | TGGTATTATTTCATAGCCCTTC | ATGGTATTGTTGGATGGTGT | 55 | 272 |
| mMaCIR162 | AM950452 | (CA)8 | 11 | CTGCCTGTCCCACGA | GCGGCCATCATAATTCC | 57 | 161 |
| mMaCIR163 | AM950453 | (AC)14 | 1 | TGAAACAATCTTCATCAGCT | TCTGGACTTGGATGCTATTT | 55 | 247 |
| mMaCIR167 | AM950367 | (AC)7, | 1 | CACTTCCACCTCTGCATC | GGTCTACTAACTTGAACACGAAC | 55 | 336 |
| mMaCIR168 | AM950368 | (CA)7, | 10 | GCACCAAACCAGTCCTAC | CGTCTCAGTTGCCGTG | 55 | 243 |
| mMaCIR169 | AM950369 | (CT)14,(CA)1 | 3 | TTTGGAGGAGACCATGATT | GCATTACATATCCTGCCTTT | 55 | 297 |
| mMaCIR170 | AM950370 | (CA)8, | 3 | GGGCCTCCATAAGCAA | ACTTACCTTCCTGCCCAC | 55 | 202 |
| mMaCIR171 | AM950371 | (CA)5,(GA)10 | 7 | GTAATACAAGTCTTCAGAGCAT | CTGTTTCGCCACTATCTT | 51 | 192 |
| mMaCIR172 | AM950372 | (CT)19, | 1 | CAGCTAATGCCAAACCC | CGACTTCGAGCGAGC | 55 | 258 |
| mMaCIR174 | AM950374 | (AG)13, | 2 | GAACCCACCTCCCTCTT | TGGGATTCCTGAGTGCT | 55 | 167 |
| mMaCIR180 | AM950380 | (CA)7, | 1 | GCCTCAGCCTCATCATC | CACCCACTCGACCCA | 55 | 226 |
| mMaCIR182 | AM950382 | (TC)22, | 9 | AACGCTTCTGCCTTGTT | TGAGACGTATTGCCCTAGTT | 55 | 150 |
| mMaCIR184 | AM950384 | (TG)7, | 3 | TGTCATCGGCATAGACTG | TGGAATTGAACTGAAGCC | 54 | 314 |
| mMaCIR185 | AM950385 | (TG)8, | 2 | CATTTCTATTCCCAGTCCC | CCAATGTTACTTCCATGCT | 54 | 181 |
| mMaCIR188 | AM950388 | (TG)9,(TA)7, | 3 | GTGCTTGTTCGCTTGTTT | AGCCCAAGTATCCCACC | 56 | 160 |
| mMaCIR189 | AM950389 | (CT)3,(CT)16 | 2 | GGGAGGGCAGAGGAA | GCCGAACTTGGTAATGTG | 55 | 259 |
| mMaCIR192 | AM950458 | (TG)8 | 3 | TGACCTAGCACAACGCA | GCTTATGTTTCATCGCCTT | 56 | 133 |
| mMaCIR193 | AM950459 | (AC)8 | 9 | TGTCCCTATCTGTCCTCTTT | CGCTTTGGAGTGTGCT | 54 | 301 |
| mMaCIR195 | AM950461 | (GA)11,(GA)6 | 5 | GAATCGCCTTAGTCTCACC | TCATGTGCTCCCATCTTT | 55 | 285 |
| mMaCIR196 | AM950462 | (TA)4,(TC)17,(TC)3 | 7 | GCTCCAAACCTCCCTTT | CGATGCCACACTGGAC | 55 | 173 |
| mMaCIR210 | AM950476 | (GA)3,(TG)12,(AG)5 | 7 | GGAAGGTGGCATGAAAG | TAACCTGATACCCATGTATTGA | 55 | 319 |
| mMaCIR214 | AM950480 | (AC)7 | 10 | CCATTGAGAGATCAACCC | CTATTTGACGTTGGTGGTC | 54 | 107 |
| mMaCIR215 | AM950481 | (GT)7,(AT)3 | 4 | AAGTTGGAGATATAGAATGGGT | TCCAGTGAATATGGATCAGT | 54 | 327 |
| mMaCIR219 | AM950485 | (GA)18,(AC)1 | 8 | GGGTAAGCTCAAGATGGAA | CAGACGCTAAACGACACC | 55 | 320 |
| mMaCIR228 | AM950494 | (CT)18,(AC)7 | 5 | CAAGCATGTTAGTTTGGGA | AAGGTGCATCCAAGGG | 55 | 197 |
| mMaCIR229 | AM950495 | (GA)22 | 10 | CTGGGTTCCTCACCTTCT | GAAACACCATGTCCCAAA | 55 | 253 |
| mMaCIR235 | AM950501 | (CT)7,(CT)8 | 2 | CCATCCCAGGCCATA | GCCCAGAGTCCGAAAG | 55 | 329 |
| mMaCIR241 | AM950503 | (TC)20 | 3 | GCTAAGCATCAAGTAGCCC | ACGAACAAGCAATCAAAGTAG | 55 | 297 |
| mMaCIR247 | AM950395 | (GT)10, | 5 | AATGGATTGGGCATCAG | GGAGGGAGGAGGGTTT | 55 | 178 |
| mMaCIR248 | AM950509 | (TG)6,(GA)6,(AG)8 | 3 | ATGCCTGCTACCACCTC | GCAGTTCCACAGTCCAAG | 55 | 251 |
| mMaCIR249 | AM950396 | (TG)9, | 4 | TGTATTGTATCCCTAATGTCCC | CCTTACTAGCCAATTACGTGAG | 56 | 279 |
| mMaCIR252 | AM950510 | (TC)9,(TC)3 | 4 | TCGTAAGCGAAAGGTCG | CGAACGCACTACCACTATG | 56 | 180 |
| mMaCIR254 | AM950512 | (CT)25 | 9 | CATGGAGGGTTAGGAGC | ATGCTTATTCTATGGTGGTTG | 54 | 180 |
| mMaCIR256 | AM950399 | (CA)7, | 4 | TTGCGGGAAACTGCT | GTTGCACTGCCCACTT | 54 | 280 |
| mMaCIR257 | AM950400 | (CA)7, | 9 | CTTTACCGAGTTGAGGG | TCATATCAGAAGATAGCCAA | 51 | 234 |
| mMaCIR260 | AM950515 | (TG)8 | 8 | GATGTTTGGGCTGTTTCTT | AAGCAGGTCAGATTGTTCC | 55 | 189 |
| mMaCIR261 | AM950516 | (CA)13 | 6 | TATCAGGCATACGTTCTGTAG | AAAGAAGGTGGGTGATAGG | 54 | 202 |
| mMaCIR264 | AM950519 | (CT)17 | 4 | AGGAGTGGGAGCCTATTT | CTCCTCGGTCAGTCCTC | 54 | 235 |
| mMaCIR272 | AM950408 | (AC)6,(CT)5, | 11 | CTCACCGGATGGCAC | GGCATTAAGTTTCAGGAATAAG | 55 | 171 |
| mMaCIR273 | AM950521 | (TC)22,(CT)6 | 9 | TGGTTGAAGATTCCCAT | GATCAAGAGGTGACAAACC | 53 | 211 |
| mMaCIR274 | AM950409 | (AC)11, | 5 | TAGCTCTTTCAACACTCTCATC | CTGGAGGCAGCGAAC | 54 | 150 |
| mMaCIR277 | AM950523 | (TG)12 | 5 | ACGATAGGATTATTGGCTGT | GGCTCTTAATTTGACAAGAA | 54 | 212 |
| mMaCIR280 | AM950412 | (TC)7,(AC)7 | 4 | GGGTCCCTGTTGGCT | TTGCAGATTAGGGTGGG | 55 | 221 |
| mMaCIR282 | AM950414 | (AG)8,(AG)3,(TG) | 9 | CATCCTGTTGCTCCCTC | AAGAATCTAGCAGCATCCAA | 55 | 209 |
| mMaCIR285 | AM950416 | (TC)21,(AC)7,(AC)5 | 2 | ATTGCCATGATTGACCC | TACGGCTCCTATCGTCC | 55 | 183 |
| mMaCIR287 | AM950526 | (TG)7 | 10 | TTTAAGAATCCCTCGCTTT | ACAGATGACGAACAAACTACC | 54 | 203 |
| mMaCIR289 | AM950418 | (GT)8,(TC)3, | 3 | TTGCTTCCTGTAACATCTCC | GGTCTGGGTGAAGGCA | 56 | 205 |
| mMaCIR294 | AM950421 | (AC)7,(TC)8,(CA)5, | 10 | CACGAGTCATAATCCAGTCA | GTTCAAAGCTCGTTGGG | 55 | 178 |
| mMaCIR297 | AM950424 | (TC)9,(AC)13,(CA)9 | 11 | GAACTCGGATTGTTCCTTT | AGGCTGATGGTAGCGAG | 55 | 173 |
| mMaCIR301 | AM950427 | (TG)11, | 6 | CATGATGTTTGAGTTTGC | CTGGAAAGCAACACCG | 54 | 166 |
| mMaCIR305 | AM950429 | (CA)5,(TC)6,(GA)3, | 3 | CCGATCAATTCAGCCA | TATGAGCAAGAACAGCCC | 55 | 299 |
| mMECIR0494 | pCav22 | (TG)6c(GT)12 | 9 | CCATGATACGGGCTTACGA | TCAATTACCAGCATCCTTACTT | 56 | 266 |
| mMECIR0496 | ATPVScl3 | (TA)7 | 4 | CGCCACATAAGGCTCCCT | GTCGCCATCTCCTTGAA | 55 | 176 |
| mMECIR0498 | SSHBSVban9a08 | (GT)8nnnnn(TTC)13 | 6 | CGGGGTCGTGTCTTAGGAA | GCAATCACACGGATACCTC | 56 | 183 |
| mMECIR0499 | SSHBSVban9a08 | (AG)8) | 1 | CGCTTGCCTTTGGTTGTG | CCAGTAGACGCCAATGC | 56 | 172 |
| mMECIR0500 | SSH didier cl3 | (AG)9 | 11 | CCAGCAGACGCACACAAA | GCAACTGCAAATGAGGG | 56 | 150 |
Calculated primer annealing temperature (Tm) and expected product size in reference variety (i.e. M. acuminata 'Calcutta 4 and M. balbisiana "Pisang Klutuk Wulung" for mMaCIR102 to mMaCIR305 and M. acuminata 'Cavendish' for mMECIR0494 to mMECIR0500). Expected size does not take into account the M13 tail (19 bp) added to each 5'end of forward primer. LG: linkage group; Ann: Annealing temperature used.