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. 2010 Apr 13;10:65. doi: 10.1186/1471-2229-10-65

Table 2.

Characteristics of the mapped SSR loci from a Musa genomic library (mMaCIR102 to mMaCIR 305) and from EST sequences (mMECIR0494 to mMECIR0500).

SSR EMBL motif LG forward primer reverse primer Ann (°C) Size (bp)
mMaCIR102 AM950328 (AG)10,(TG)5 6 TGTTGGATTGGCTTCATC CTTCGTTCAATGGTCTCCT 55 220
mMaCIR103 AM950329 (CT)14, 3 CCTCTTCTCCCTGTGTTG CGGTTTAACATACCTATTCTTG 54 179
mMaCIR105 AM950331 (CA)8,(CT)15 6 CATCCACTTGCTTTTCCA CTTCACGGCTTCCACA 56 264
mMaCIR108 AM950334 (CA)7,(CA)4, 2 ACGCATGGTAAAGTGGAA ACATTCAAATCACGTTGCT 55 111
mMaCIR109 AM950335 (CA)13, 4 ACTCTAGTTCCAGAATAACTCCA CAATCTTCATTAGCCAGTTGT 55 204
mMaCIR110 AM950336 (AC)7,(GA)6, 3 GGTGAACTGATGTGCGA TCTTTCAACGGAATAAGCA 55 244
mMaCIR111 AM950337 (CA)8, 6 TCGTATGGAACAACAGTCC CTTTCACCTTCAAACAGCA 55 137
mMaCIR112 AM950338 (CA)5,(CA)15 7 GTTCGGCTGGAGGTAGTT AAGAACACGAAGGCAGG 55 330
mMaCIR113 AM950339 (CA)10, 10 TCAAGTATTTCACCGTATTGC TTACCACCCTGTCATCTTTC 55 207
mMaCIR114 AM950340 (AC)7,(CT)28 8 GCAAGCCAAAGGGAA ACCAACAAAGAATGGTGTAA 54 222
mMaCIR115 AM950341 (CA)2, 11 CAAGAGACTACCACCGAAGA TGATTCTCACGACGTATGG 55 114
mMaCIR116 AM950342 (TC)2,(TC)20 11 ACACAAAGAAACCAGCCA CGTCCCATCGTCTCCT 55 202
mMaCIR117 AM950343 (TC)20, 7 GTTTGTGGAATAAGTGGGAA ATGAGGGAGTTAGTGGTGG 55 214
mMaCIR119 AM950345 (CA)9,(TA)6,(CA)5, 10 TGAAAAGCAATCCAACCT ACCCTGAAATGTTTGTCTTT 54 395
mMaCIR122 AM950348 (GT)8, 7 CGGTGACACTGGAAGGT CAACTGAAGAACTGCCACTAA 56 204
mMaCIR124 AM950350 (AC)7, 9 ACCTTGACAGCCCTCTTC ATCAATCATTTCTGGGGTT 55 63
mMaCIR129 AM950355 (CA)6, 5 CTAACCTTTGATTCTGTTTG GTCCCTGATACACCATTC 50 214
mMaCIR130 AM950356 (TG)17, 6 TTAAACGTCTCCGTGTCTTC TTGCATGAGGCTGGG 56 311
mMaCIR137 AM950363 (TC)12, 4 CGTATTCTACATCTGCTTCTTT GCAGTGATTAGGTGATGATTT 54 223
mMaCIR138 AM950364 (CA)7, 3 TCATTCTCATGCGGAACT CGGTGGATGTTGTTGG 55 173
mMaCIR139 AM950365 (GA)18, 5 TCGTCCCCTACTGCCT ATGCTTCCGTTTGGCT 55 187
mMaCIR150 AM950440 (CA)10 3 ATGCTGTCATTGCCTTGT GAATGCTGATACCTCTTTGG 55 238
mMaCIR151 AM950441 (CT)21 3 TATCCACCTCCTGGCAC GCCAAACATCACCCAAC 55 172
mMaCIR152 AM950442 (CTT)18,(CT)17,(CA)6 4 CCACCTTTGAGTTCTCTCC TTTCCCTCTTCGATTCTGT 55 163
mMaCIR154 AM950444 (CT)17 4 CATTCAGCATGGAAACCT CTTCCTCAAACTGCTCCTC 55 311
mMaCIR156 AM950446 (TG)23 4 CTTTCTGAAGGAAATTCTGAC AGTGCAGCCCAATGAA 54 210
mMaCIR157 AM950447 (CA)9,(TA)7 11 TGGTATTATTTCATAGCCCTTC ATGGTATTGTTGGATGGTGT 55 272
mMaCIR162 AM950452 (CA)8 11 CTGCCTGTCCCACGA GCGGCCATCATAATTCC 57 161
mMaCIR163 AM950453 (AC)14 1 TGAAACAATCTTCATCAGCT TCTGGACTTGGATGCTATTT 55 247
mMaCIR167 AM950367 (AC)7, 1 CACTTCCACCTCTGCATC GGTCTACTAACTTGAACACGAAC 55 336
mMaCIR168 AM950368 (CA)7, 10 GCACCAAACCAGTCCTAC CGTCTCAGTTGCCGTG 55 243
mMaCIR169 AM950369 (CT)14,(CA)1 3 TTTGGAGGAGACCATGATT GCATTACATATCCTGCCTTT 55 297
mMaCIR170 AM950370 (CA)8, 3 GGGCCTCCATAAGCAA ACTTACCTTCCTGCCCAC 55 202
mMaCIR171 AM950371 (CA)5,(GA)10 7 GTAATACAAGTCTTCAGAGCAT CTGTTTCGCCACTATCTT 51 192
mMaCIR172 AM950372 (CT)19, 1 CAGCTAATGCCAAACCC CGACTTCGAGCGAGC 55 258
mMaCIR174 AM950374 (AG)13, 2 GAACCCACCTCCCTCTT TGGGATTCCTGAGTGCT 55 167
mMaCIR180 AM950380 (CA)7, 1 GCCTCAGCCTCATCATC CACCCACTCGACCCA 55 226
mMaCIR182 AM950382 (TC)22, 9 AACGCTTCTGCCTTGTT TGAGACGTATTGCCCTAGTT 55 150
mMaCIR184 AM950384 (TG)7, 3 TGTCATCGGCATAGACTG TGGAATTGAACTGAAGCC 54 314
mMaCIR185 AM950385 (TG)8, 2 CATTTCTATTCCCAGTCCC CCAATGTTACTTCCATGCT 54 181
mMaCIR188 AM950388 (TG)9,(TA)7, 3 GTGCTTGTTCGCTTGTTT AGCCCAAGTATCCCACC 56 160
mMaCIR189 AM950389 (CT)3,(CT)16 2 GGGAGGGCAGAGGAA GCCGAACTTGGTAATGTG 55 259
mMaCIR192 AM950458 (TG)8 3 TGACCTAGCACAACGCA GCTTATGTTTCATCGCCTT 56 133
mMaCIR193 AM950459 (AC)8 9 TGTCCCTATCTGTCCTCTTT CGCTTTGGAGTGTGCT 54 301
mMaCIR195 AM950461 (GA)11,(GA)6 5 GAATCGCCTTAGTCTCACC TCATGTGCTCCCATCTTT 55 285
mMaCIR196 AM950462 (TA)4,(TC)17,(TC)3 7 GCTCCAAACCTCCCTTT CGATGCCACACTGGAC 55 173
mMaCIR210 AM950476 (GA)3,(TG)12,(AG)5 7 GGAAGGTGGCATGAAAG TAACCTGATACCCATGTATTGA 55 319
mMaCIR214 AM950480 (AC)7 10 CCATTGAGAGATCAACCC CTATTTGACGTTGGTGGTC 54 107
mMaCIR215 AM950481 (GT)7,(AT)3 4 AAGTTGGAGATATAGAATGGGT TCCAGTGAATATGGATCAGT 54 327
mMaCIR219 AM950485 (GA)18,(AC)1 8 GGGTAAGCTCAAGATGGAA CAGACGCTAAACGACACC 55 320
mMaCIR228 AM950494 (CT)18,(AC)7 5 CAAGCATGTTAGTTTGGGA AAGGTGCATCCAAGGG 55 197
mMaCIR229 AM950495 (GA)22 10 CTGGGTTCCTCACCTTCT GAAACACCATGTCCCAAA 55 253
mMaCIR235 AM950501 (CT)7,(CT)8 2 CCATCCCAGGCCATA GCCCAGAGTCCGAAAG 55 329
mMaCIR241 AM950503 (TC)20 3 GCTAAGCATCAAGTAGCCC ACGAACAAGCAATCAAAGTAG 55 297
mMaCIR247 AM950395 (GT)10, 5 AATGGATTGGGCATCAG GGAGGGAGGAGGGTTT 55 178
mMaCIR248 AM950509 (TG)6,(GA)6,(AG)8 3 ATGCCTGCTACCACCTC GCAGTTCCACAGTCCAAG 55 251
mMaCIR249 AM950396 (TG)9, 4 TGTATTGTATCCCTAATGTCCC CCTTACTAGCCAATTACGTGAG 56 279
mMaCIR252 AM950510 (TC)9,(TC)3 4 TCGTAAGCGAAAGGTCG CGAACGCACTACCACTATG 56 180
mMaCIR254 AM950512 (CT)25 9 CATGGAGGGTTAGGAGC ATGCTTATTCTATGGTGGTTG 54 180
mMaCIR256 AM950399 (CA)7, 4 TTGCGGGAAACTGCT GTTGCACTGCCCACTT 54 280
mMaCIR257 AM950400 (CA)7, 9 CTTTACCGAGTTGAGGG TCATATCAGAAGATAGCCAA 51 234
mMaCIR260 AM950515 (TG)8 8 GATGTTTGGGCTGTTTCTT AAGCAGGTCAGATTGTTCC 55 189
mMaCIR261 AM950516 (CA)13 6 TATCAGGCATACGTTCTGTAG AAAGAAGGTGGGTGATAGG 54 202
mMaCIR264 AM950519 (CT)17 4 AGGAGTGGGAGCCTATTT CTCCTCGGTCAGTCCTC 54 235
mMaCIR272 AM950408 (AC)6,(CT)5, 11 CTCACCGGATGGCAC GGCATTAAGTTTCAGGAATAAG 55 171
mMaCIR273 AM950521 (TC)22,(CT)6 9 TGGTTGAAGATTCCCAT GATCAAGAGGTGACAAACC 53 211
mMaCIR274 AM950409 (AC)11, 5 TAGCTCTTTCAACACTCTCATC CTGGAGGCAGCGAAC 54 150
mMaCIR277 AM950523 (TG)12 5 ACGATAGGATTATTGGCTGT GGCTCTTAATTTGACAAGAA 54 212
mMaCIR280 AM950412 (TC)7,(AC)7 4 GGGTCCCTGTTGGCT TTGCAGATTAGGGTGGG 55 221
mMaCIR282 AM950414 (AG)8,(AG)3,(TG) 9 CATCCTGTTGCTCCCTC AAGAATCTAGCAGCATCCAA 55 209
mMaCIR285 AM950416 (TC)21,(AC)7,(AC)5 2 ATTGCCATGATTGACCC TACGGCTCCTATCGTCC 55 183
mMaCIR287 AM950526 (TG)7 10 TTTAAGAATCCCTCGCTTT ACAGATGACGAACAAACTACC 54 203
mMaCIR289 AM950418 (GT)8,(TC)3, 3 TTGCTTCCTGTAACATCTCC GGTCTGGGTGAAGGCA 56 205
mMaCIR294 AM950421 (AC)7,(TC)8,(CA)5, 10 CACGAGTCATAATCCAGTCA GTTCAAAGCTCGTTGGG 55 178
mMaCIR297 AM950424 (TC)9,(AC)13,(CA)9 11 GAACTCGGATTGTTCCTTT AGGCTGATGGTAGCGAG 55 173
mMaCIR301 AM950427 (TG)11, 6 CATGATGTTTGAGTTTGC CTGGAAAGCAACACCG 54 166
mMaCIR305 AM950429 (CA)5,(TC)6,(GA)3, 3 CCGATCAATTCAGCCA TATGAGCAAGAACAGCCC 55 299
mMECIR0494 pCav22 (TG)6c(GT)12 9 CCATGATACGGGCTTACGA TCAATTACCAGCATCCTTACTT 56 266
mMECIR0496 ATPVScl3 (TA)7 4 CGCCACATAAGGCTCCCT GTCGCCATCTCCTTGAA 55 176
mMECIR0498 SSHBSVban9a08 (GT)8nnnnn(TTC)13 6 CGGGGTCGTGTCTTAGGAA GCAATCACACGGATACCTC 56 183
mMECIR0499 SSHBSVban9a08 (AG)8) 1 CGCTTGCCTTTGGTTGTG CCAGTAGACGCCAATGC 56 172
mMECIR0500 SSH didier cl3 (AG)9 11 CCAGCAGACGCACACAAA GCAACTGCAAATGAGGG 56 150

Calculated primer annealing temperature (Tm) and expected product size in reference variety (i.e. M. acuminata 'Calcutta 4 and M. balbisiana "Pisang Klutuk Wulung" for mMaCIR102 to mMaCIR305 and M. acuminata 'Cavendish' for mMECIR0494 to mMECIR0500). Expected size does not take into account the M13 tail (19 bp) added to each 5'end of forward primer. LG: linkage group; Ann: Annealing temperature used.