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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6697–6708. doi: 10.4049/jimmunol.0800997

FIGURE 2.

FIGURE 2

Chronic Ag stimulation reduced the quantity of day-30 virus-specific CD8+ T cells. OT-I transferred C57BL/6 mice were single or chronic stimulated with WSN-OVA. On day 30, the frequency of virus-specific CD8+ T cells was determined by flow cytometry. A, Representative flow cytometry virus-specific CD8+ T cells (gated on CD8+ T cells). B, Reduction in virus-specific CD8+ T cells was observed in pooled frequency and absolute number (n = 15 mice/group, p < 0.001). C, Kinetic analysis of virus-specific OT-I cells during 30 days of priming (n = 3 mice/group). D, Reduction of chronic CD8+ T cells in lymphoid and nonlymphoid tissues indicates migration is not impaired.