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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6697–6708. doi: 10.4049/jimmunol.0800997

FIGURE 4.

FIGURE 4

Chronic Ag stimulation reduced the quality of virus-specific CD8+ T cells. An equal number of single or chronic Ag-stimulated day-30 virus-specific CD8+ T cells were transferred to naive C57BL/6 mice. Recipient mice were i.n. challenged with 40 TCID50 WSN-OVA and harvested at the peak of the secondary response. Representative flow cytometry plots (A) and pooled frequencies and absolute numbers (B), showing a significant decrease in virus-specific CD8+ T cells in the lung of chronic mice compared with single prime mice. Each symbol represents a single animal. Representative flow cytometry plots (C) and pooled frequencies and absolute numbers (D) show that chronic Ag stimulation with PR8 also results in a significant reduction in responding virus-specific CD8+ T cells relative to single prime controls, following i.n. viral rechallenge.