FIGURE 5.

Comparative analysis of CD11b expression and PMN migration during primary infection of RMs with the pathogenic SIVmac251 strain (n = 6), or the attenuated SIVΔnef strain (n = 4) and AGMs (n = 5). A, Whole blood samples from SIVΔnef, SIV⁺ RMs (slow progressors, RM SP, and moderate progressors, RM MP) and AGMs were incubated with PBS or fMLP (10⁻⁶M) at 37°C for 5 min and then stained with PE-anti-CD11b Ab at 4°C for 30 min. The results are expressed as a stimulation index (mean fluorescence intensity of the sample incubated with fMLP/MFI of the sample incubated with PBS). B and C, PMN migration toward fMLP (10⁻⁷ M) (B) or IL-8 (25 ng/ml) (C) was measured in Trans-well plates as described in Materials and Methods. The results are expressed as the migration rate: (number of PMN in the lower well after migration/number of PMN applied to the upper well before migration) × 100. *, p < 0.05, Significantly different from SIVΔnef macaques; †, p < 0.05, significantly different from RM slow progressors.