. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: Horm Behav. 2010 Mar 27;58(3):355–367. doi: 10.1016/j.yhbeh.2010.03.013

Table 1.

Methods used for real time rt-PCR.

Gene	Size (bp)	Primer	Sequence (5' to 3')	Annealing (°C)	Melting peak (°C)	Reference
Orexin	143	sense	CATCCTCACTCTGGGAAA	60	88	(Teske et al., 2006b)
Orexin	143	antisense	AGGGATATGGCTCTAGCT	60	88	(Teske et al., 2006b)
OXR1	244	sense	AGAGAGCAGAGAGCGTTGTAAACC	60	84	(Teske et al., 2006b)
OXR1	244	antisense	TTCACAGGGACACATTGGTGC	60	84	(Teske et al., 2006b)
OXR2	257	sense	TGTTCAAGAGCACAGCCAAACG	60	82	(Teske et al., 2006b)
OXR2	257	antisense	GCCAATACCATAAGACACAGGGG	60	82	(Teske et al., 2006b)
CRF	150	sense	CTCTCTGGATCTCACCTTCCAC	60	80	(Chance et al., 2007)
CRF	150	antisense	CTAAATGCAGAATCGTTTTGGC	60	80	(Chance et al., 2007)
Snark	294	sense	CTGTACATCCTGGTGCATGG	60	85.5	NM 001007617
Snark	294	antisense	ATGACCTTGCCCTTATGCT	60	85.5	NM 001007617
PEPCK	252	sense	GAGACCACAGGATGAGGAA	60	85	(Azzout-Marniche et al., 2007)
PEPCK	252	antisense	ATGACCTTGCCCTTATGCT	60	85	(Azzout-Marniche et al., 2007)
L32	122	sense	CGGAAGTTTCTGGTCCACAATGTC	60	79	(Teske et al., 2006b)
L32	122	antisense	GCTCTTTCTACGATGGCTTTTCGG	60	79	(Teske et al., 2006b)