. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: Horm Behav. 2010 Mar 27;58(3):355–367. doi: 10.1016/j.yhbeh.2010.03.013

Table 6.

Intra-PVN orexin-A-induced increases in activity and energy expenditure in high- and low-capacity rats (HCR and LCR) before and after one month on a high-fat diet. Mean ± SEM.

			Energy Expenditure				Activity (counts/min)
strain	diet	Orexin-A (nmoles)	VO₂ (ml/kg/hr)	EE (kcal/hr)	EE (kcal/kg^0.75/hr)	RER	vertical	ambulatory	stationary	% time active
HCR	regular	vehicle	1285±92^‡	2.25±0.19^‡	0.030±0.002	0.91±0.03	0.31±0.17	8±3	9±3	52±8
		0.125	1636±98^‡^*	2.86±0.26^*	0.035±0.002^*	0.88±0.02^‡	2.55±0.53^*	36±5^*	41±7^*^‡	94±4^*
		0.25	1698±112^‡^*	3.00±0.16^*^‡	0.036±0.005^*	0.91±0.04	3.15±1.64^*	35±5^*	42±8^*	99±1^*
		0.5	1654±132^‡^*	2.85±0.25^*^‡	0.035±0.006^*	0.86±0.03	2.45±0.85^*	40±9^*	43±8^*	98±2^*
		1.0	1605±111^‡^*	2.74±0.20^*^‡	0.034±0.002^*	0.85±0.02	1.86±0.47^*	33±6^*	42±7^*	98±0.6^*
	high-fat	vehicle	1372±64^‡	2.59±0.19	0.029±0.001^‡	0.77±0.01^‡	0.47±0.27^*	11±4^*	14±4^*	65±11^*
		0.125	1614±21^‡^*	3.06±0.20^*^‡	0.034±0.001^*	0.79±0.01^†	1.94±0.76^*	27±6^*	37±11^*	97±1^*
		0.25	1841±108^‡^*	3.53±0.29^*	0.039±0.002^*^‡	0.79±0.02^*^†	3.87±0.72^*^‡	37±10^*	38±12^*	97±1^*
		0.5	1720±91^‡^*	3.24±0.21^*	0.037±0.002^*	0.80±0.01^†	3.51±0.38^*^‡	43±5^*^‡	51±9^*^‡	96±4^*
		1.0	1860±113^‡^*	3.50±0.25^*	0.040±0.002^*^‡	0.80±0.01^†	3.25±0.43^†^‡	48±5^*^†^‡	53±5^*^‡	100±0^*^†
LCR	regular	vehicle	1117±26	2.83±0.15	0.026±0.001	0.90±0.02	0.45±0.24	13±3	19±5	70±6
		0.125	1308±56^*	3.52±0.17^*	0.030±0.001^*	0.86±0.02	1.50±0.30^*	21±3.43^*	24±4^*	88±3^*
		0.25	1369±38^*	3.56±0.08^*	0.033±0.001^*	0.87±0.01	1.61±0.35^*	31±4^*	33±4^*	95±3^*
		0.5	1423±55^*	3.55±0.19^*	0.033±0.001^*	0.85±0.02^*	2.42±0.56^*	38±6^*	38±4^*	97±3^*
		1.0	1370±56^*	3.48±0.20^*	0.032±0.001^*	0.89±0.02	2.22±0.50^*	37±5^*	38±4^*	99±1^*
	high-fat	vehicle	1011±63	2.99±0.15	0.024±0.001	0.79±0.01^†	0.23±0.12	12±3	15±3	75±4
		0.125	1201±41^*^†	3.55±0.13^*^†	0.028±0.001^*	0.78±0.01^†	1.10±0.53^*	22±2^*	24±3^*	92±6^*
		0.25	1293±65^*	3.80±0.2^*^†	0.030±0.001^*	0.79±0.01^†	1.11±.032^*	29±5^*	28±4^*	92±4^*
		0.5	1231±63^*^†	3.68±0.24^*	0.029±0.002^*^†	0.79±0.01^†	1.33±0.27^*^†	29±5^*^†	30±4^*^†	92±3^*
		1.0	1304±70^*	3.92±0.67^*^†	0.031±0.002^*	0.78±0.01^†	1.50±0.29^*^†	34±6^*	28±3^*^†	96±2^*

HCR n=5; LCR n=9.

different from vehicle value in same group on same diet (p<0.05).

^†

different from same dose in same group on regular diet (p<0.05).

^‡

different from corresponding data point (same diet and dose) in LCR group (p<0.05).