Table 7.
mRNA levels measured using real-time rt-PCR.
| Tissue | Gene | Site | Fold change, compared to HCR on the regular diet | |||
|---|---|---|---|---|---|---|
| Regular Diet | High-Fat Diet | |||||
| HCR | LCR | HCR | LCR | |||
| Brain | preproorexin | PeFLH | 1.0 | 1.3 | 2.0 | 2.2 |
|
| ||||||
| OXR1 | PeFLH | 1.0 | 1.1 | 1.1 | 11.1 | |
|
| ||||||
| PVN | 1.0 | 1.1 | −1.2 | 1.3 | ||
|
| ||||||
| PVT | 1.0 | 1.1 | −1.0 | 1.7 | ||
|
| ||||||
| OXR2 | PeFLH | 1.0 | 1.4 | 1.3 | 1.3 | |
|
| ||||||
| PVN | 1.0 | 1.3 | −1.0 | 1.2 | ||
|
| ||||||
| PVT | 1.0 | 1.1 | −1.0 | 1.3 | ||
|
| ||||||
| CRH | PVN | 1.0 | 1.2 | −1.3 | −1.3 | |
|
| ||||||
| Snark | PeFLH | 1.0 | 1.6 | 1.3 | 1.5 | |
|
| ||||||
| Arc | 1.0 | 1.1 | 1.1 | 1.3 | ||
|
| ||||||
| PVN | 1.0 | −1 | 1.1 | 1.5 | ||
|
| ||||||
| Skeletal muscle | PEPCK-C | gastrocnemius | 1.0 | 3.3 | 1.2 | −2.1 |
|
| ||||||
| soleus | 1.0 | 2.1 | −1.8 | 2.5 | ||
Orexin receptor 1 (OXR1) and 2 (OXR2); perifornical lateral hypothalamus (PeFLH), Paraventricular nucleus of the hypothalamus (PVN), Paraventricular nucleus of the thalamus (PVT), Arcuate nucleus (Arc). (Backberg et al., 2002; Marcus et al., 2001)