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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: Can J Physiol Pharmacol. 2008 Jul;86(7):438–448. doi: 10.1139/y08-054

Fig. 2.

Fig. 2

NPY-induced VSMC proliferation is mediated by Gi/o proteins. (A) Pertussis toxin (PTX) (100 ng·mL−1, pretreatment for 6 h) blocked the proliferative effect of NPY in rat aortic VSMCs, measured as an increase in [3H]thymidine uptake, at both high- and low-affinity growth peaks. Significant at *, p < 0.05 compared with control using two-way ANOVA followed by Tukey's test. #, p< 0.05 compared with samples treated with NPY only at the same concentration. n = 3 separate experiments. (B) NPY, at all concentrations, inhibited forskolin-stimulated increases in cAMP levels in VSMCs. VSMCs were incubated with IBMX (10−4 mol·L−1) for 5 min, then treated with NPY and forskolin (10−6 mol·L−1) in 0.25% FBS SmBM for 60 min; intracellular cAMP concentrations were measured by ELISA. Significant at *, p < 0.05 compared with forskolin and IBMX alone by one-way RM ANOVA followed by Dunnett's t test. n = 3 separate experiments, 5 wells per experiment.