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. 2010 Aug 18;5(8):e12229. doi: 10.1371/journal.pone.0012229

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Experiments were conducted as described under Materials and Methods. Photographs of ECL-stained immunoblots are shown. A, Immunoprecipitations (IP) of MEF cell extracts with anti-AAG antibody or control non-immune IgG: Top panel, immunoblotting to detect pol β; bottom panel, immunoblotting to detect AAG. In the top panel, IP incubations were performed using matched wild-type (AAG +/+) (lanes 1 and 2) and AAG −/− cell extracts (lane 3) and anti-AAG or non-immune IgG (lane 2); immonoblotting was with anti-pol β antibody. In the bottom panel, immunoblotting was with anti-AAG antibody. In lane 4, 50 µg of AAG +/+ extract (1/20^th of the IP input) was subject to SDS-PAGE as a marker for AAG. B, Immunoprecipitations of MEF cell extracts as in A with anti-pol β antibody or non-immune IgG. Top panel, immunoblotting to detect AAG; bottom panel, immunoblotting to detect pol β. Panels C and D illustrate pol λ and OGG1 co-immunoprecipitation from MEF cell extracts, as in panels A and B. IP incubations were performed with matched pol λ+/+ cell extract (lanes 1 and 2) and pol λ−/− cell extract (lane 3), as shown in the figure. Similarly, non-immune IgG was used as a negative control (lanes 2). C. Top panel, immunoblotting to detect OGG1; bottom panel, immunoblotting to detect pol λ. D. Top panel, immunoblotting to detect pol λ; bottom panel, immunoblotting to detect OGG1. In lane 4, 50 µg of pol λ+/+ extract was subject to SDS-PAGE as a marker.