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. 2010 Aug 18;5(8):e12224. doi: 10.1371/journal.pone.0012224

Figure 1. Mechanical steps.

Figure 1

Typical traces of time courses for M5SH steps at 100 µM ATP (A) and 10 µM ATP (B), and for M5DH at 100 µM ATP (C) and 10 (D) µM ATP. Gray dots represent raw data. White arrowheads show non-successive strokes; yellow arrowheads, first strokes during the successive-multisteps; blue arrowheads, successive steps; red arrowheads, backward steps. Medium: 120 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.2 mg/ml calmodulin and 20 mM Hepes (pH 7.8). Trap stiffness, 0.02–0.025 pN/nm. Temperature, 25°C.