Figure 3. Size of successive steps.

(A, B) Histogram of successive steps for M5SH (A) and M5DH (B) in 10 µM ATP. Lines are Gaussian distribution fits. Arrowheads indicate peak values, 32±13 and −36±17 nm (A), and 34±15 and −30±13 nm (B) (mean ± S.D), respectively. (C, D) ATP dependency for forward (C) and backward (D) stepsize during successive steps. Red circles and blue squares indicate M5SH (C, n = 20–100; D, n = 18–36) and M5DH (C, n = 60–115; D, n = 19–24), respectively. Red lines are average stepsizes for forward, 36.3 nm, and backward, −36.1 nm, steps. Error bars in C and D indicate standard errors.