(A, B) Force dependencies of forward (A) and backward (B) successive steps. Red circles indicate data for M5SH (n = 15–59 in A, 9–18 in B), blue squares indicate data for M5DH (A, n = 19–31; B, n = 12–16). Broken lines are average values (A, 35.4 nm; B, −36.5 nm). All data were obtained at 10 µM ATP. (C) Force dependencies for dwell time of M5SH (red circles) and M5DH (blue squares). Each point represents the average dwell time at 100 µM ATP (solid: n = 15–59 for M5SH; 20–67 for M5DH) and 10µM (open: n = 30–37 for M5SH; 18–37 for M5DH). The curves were obtained from equation (1). (D) Ratio of forward to backward movements for M5SH (red circles: n = 14–47) and M5DH (blue squares: n = 36–47). The solid lines were obtained from equation (3). NF is the number of forward steps; NB, backward steps.