Table 1. Summary of experiments.

		M5SH	M5DH
No. of cofilaments tested		509	166
No. of cofilaments interacting with actin filaments		55	20
No. of cofilaments showing successive steps		37	20
Non-successive steps	Stepsize (nm)	23±20	22±16
	Dwell time(s)*	0.45,	0.49,
		0.10	0.10
	Vmax (s−1)	11	N.D.
	Km (µM)	36	N.D.
First step during successive steps	Stepsize (nm)	20±16	22±17
	Dwell time(s)*	0.50,	0.44,
		0.10	0.11
	Vmax (s−1)	11	N.D.
	Km (µM)	28	N.D.
Successive steps	Stepsize (nm)†	32±13	34±15
		−36±17	−30±13
	Dwell time(s)*	0.40,	0.39,
		0.11	0.18
	Vmax (s−1)	10	N.D.
	Km (µM)	30	N.D.
Load dependency of directionality	Energy difference (kBT)	3.7	3.4
	Characteristic distance (nm)	5.7	4.5
	Stall force (pN)	0.8	2.2
Successivity		0.28	0.5

All errors are SD.

*100 µM ATP, 10 µM ATP, respectively.

^†Forward and backward, respectively.