Skip to main content
. 2010 Aug 18;5(8):e12271. doi: 10.1371/journal.pone.0012271

Figure 3. In vitro formation of sense-antisense fusions.

Figure 3

A: Proposed mechanism of sense-antisense fusion formation. B: Schematic of SPT7 RNA showing primer binding sites and observed sense-antisense fusions. C: Purified SPT7 substrate. D: RT-PCR experiments performed on SPT7 substrate performed as in Fig. 2C. Upper panel shows a 32 cycle PCR reaction, other panels show 25 cycles. E: Schematic of SPT7 RNA showing primer binding sites and observed exon shuffling events. F: RT-PCR experiments performed as in d. Sequenced bands are indicated by *.