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. Author manuscript; available in PMC: 2011 Aug 17.
Published in final edited form as: Dev Cell. 2010 Aug 17;19(2):220–231. doi: 10.1016/j.devcel.2010.07.007

Figure 2. β-catenin and RNA Pol II establish H3K4me3 at poised promoters.

Figure 2

(A) ChIP for H3K4me3 in 1000-cell control and β-catenin depleted (β-MO) embryos. All sibling β-MO embryos were completely ventralized at later stages (not shown). (B) H3K4me3, but not H3K9/14ac, requires β-catenin function. MBT-stage control (upper panel for each gene) and ΔNTcf3 mRNA-injected (500pg, lower panels) embryos were subjected to ChIP for β-catenin, H3K9/14ac, and H3K4me3. (C) 1000-cell stage control and β-catenin depleted (β-MO) embryos were subjected to ChIP for RNA Pol II (CTD pSer5). All sibling β-MO embryos were completely ventralized at later stages (not shown). (D) MBT-stage control (upper panels) and α-amanitin injected (10ng, lower panels) embryos were subjected to ChIP for β-catenin, H3K9/14ac, and H3K4me3.