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. Author manuscript; available in PMC: 2011 Aug 19.
Published in final edited form as: Cell Host Microbe. 2010 Aug 19;8(2):163–173. doi: 10.1016/j.chom.2010.07.006

Figure 2. Pathogen-specific CD4+ T cells primed by Lm or LCMV are heritably committed to the Th1 lineage.

Figure 2

A. CFSE-labeled CD4+ T cells from P25 TCR or SMARTA TCR transgenic mice were transferred into B6 control recipients that were subsequently infected with LmΔactA-85B or LCMV, respectively. Histograms display number of cell divisions among donor CD4+ T cells 7 days post-infection. B. Representative FACS plots indicating IFN-γ, IL-17, IL-2 and TNF-α production by naive P25, or donor P25 or SMARTA CD4+ T cells recovered day 28 after infection and stimulation with cognate peptide directly ex vivo. C. Representative FACS plots demonstrating IFN-γ and IL-17 production by donor P25 or SMARTA CD4+ T cells following culture in the indicated conditions for 5 days and re-stimulation with cognate peptide.