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. Author manuscript; available in PMC: 2011 Aug 19.
Published in final edited form as: Cell Host Microbe. 2010 Aug 19;8(2):163–173. doi: 10.1016/j.chom.2010.07.006

Figure 3. Type-I IFNs and IL-12 promote Th1 commitment among adoptively transferred pathogen-specific CD4+ T cells.

Figure 3

A. CFSE-labeled CD4+ T cells from P25 TCR transgenic mice were transferred into B6 control (WT), IFNAR-p40 DKO, or IFN-γ KO recipients that were subsequently infected with LmΔactA-85B. Histograms display number of cell divisions among adoptive transferred CD4+ T cells 7 days post-infection. B. Representative FACS plots indicating IFN-γ, IL-17, IL-2 and TNF-α production by P25 CD4+ T cells recovered day 28 after infection and stimulation with cognate peptide directly ex vivo. C. Representative FACS plots demonstrating IFN-γ and IL-17 production by P25 CD4+ T cells primed in vivo with LmΔactA-85B following culture in the indicated conditions for 5 days and re-stimulation with cognate peptide.