Figure 5. Impacts of differentiation on CD4+ T cell protective potency.
Recoverable pathogen burden in the lung day 28 after aerosol infection with 102 MTb in mice transferred P25 cells (5 × 105) one day prior to infection or no cell transfer control. “Plastic” and Th17 polarized cells were recovered from mice infected with vacuolar LmΔLLOΔPLC-85B and cultured under neutral (Tn) or Th17 conditions, respectively, for five days as described in the Experimental Procedures. Th1 cells were recovered from mice infected with cytoplasmic LmΔactA-85B and cultured under neutral (Tn) conditions. **, p < 0.01.