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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Microbiol. 2010 Mar 19;12(9):1235–1249. doi: 10.1111/j.1462-5822.2010.01465.x

Figure 3. Active kinase recruitment is not conserved in all species of chlamydiae.

Figure 3

Hela cells were infected with C. trachomatis serovars L2 or D, C. muridarum Mouse pneumonitis (MoPn), C. caviae (GPIC), or C. pneumoniae AR-39 (Cpn). At various times post infection (C. trachomatis L2, C. caviae = 24 hrs; C. trachomatis serovar D, C. muridarum = 36hrs; C. pneumoniae = 72hrs) cells were fixed, permeabilized and labeled with an species-appropriate anti-EB (green) and anti-active kinase (Tyr419) (red) antibodies. Images were acquired on a Zeiss LSM 510 Meta confocal microscope. Active kinase is recruited to the C. trachomatis and C. pneumoniae inclusions, but not to C. muridarum or C. caviae. Scale bar = 10 μm.