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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Microbiol. 2010 Mar 19;12(9):1235–1249. doi: 10.1111/j.1462-5822.2010.01465.x

Figure 6. Two microdomain Incs interact with each other.

Figure 6

T150 flasks of HeLa cells were infected with C. trachomatis L2 or mock infected. 24 hrs post infection cells were harvested and lysate prepared for co-immunoprecipitation. The supernatant from infected lysate (lanes 1, 3, 5, 7, and 9) or uninfected lysate (lanes 2, 4, 6, and 8) was added to Protein-A beads that had been pre-loaded with antibody: anti-Rickettsia negative control antibody (lane 1), anti-IncB (lanes 2 and 3), anti-Inc101 (lanes 4 and 5), anti-Inc222 (lanes 6 and 7) or anti-Inc850 (lanes 8 and 9). Bands corresponding to IncB and Inc101 are only observed in the infected IncB and Inc101 immunprecipitates respectively (lanes 3 and 5). A band corresponding to Inc222 is not observed in the anti-Rickettsia, anti-IncB, anti-Inc101 or mock infected lanes, but is observed in the anti-Inc222 and anti-Inc850 immunoprecipitated samples only (lanes 7 and 9).