. 2010 May 9;87(9):955–962. doi: 10.1007/s11746-010-1572-3

Table 1.

Mean peak area ratio of trimethylsilyl ethers

Sterols^a added	Sterols^a quantified	Saponification conditions^a
Sterols^a added	Sterols^a quantified	1M18hr24°C^b	1M18hr37°C	1M3hr45°C	3.6M3hr24°C
Cholesterol	Cholesterol tms ether	0.7502 ± 0.28 (100)	0.8730 ± 0.18 (116)	0.5468 ± 0.18 (73)	0.7855 ± 0.12 (105)
7-Ketocholesterol	7-Ketocholesterol tms ether	0.5071 ± 0.16 (100)	0.2684 ± 0.10 (53)	0.2484 ± 0.27 (49)	0.3623 ± 0.20 (71)
7-Ketocholesterol	3,5-7-One^c	0.0580 ± 0.03 (100)	0.2309 ± 0.08 (398)	0.1994 ± 0.22 (344)	0.0241 ± 0.01 (41)
β-Sitosterol	β-Sitosterol tms ether	0.5669 ± 0.29 (100)	0.6995 ± 0.26 (123)	0.3334 ± 0.21 (59)	0.5240 ± 0.25 (92)

^aSamples of cholesterol, 7-keto and β-sitosterol were saponified under the following conditions: 1 M methanolic KOH for 18 h at 24 °C (1M18hr24°C, Control), 18 h at 37 °C (1M18hr37°C), 3 h at 45 °C (1M3hr45°C), and 3.6 M methanolic KOH for 3 h at 24 °C (3.6M3hr24°C). Trimethylsilyl ethers (tms ethers) were quantified. Means (6 samples) and standard deviations with different letters are statistically different at P ≤ 0.10. Cholest-5-ene-7-one (5-ene-7-one) was used as an internal standard

^b1M18hr24°C (cold saponification) was used as the control and for comparison because it is regarded by many researchers as appropriate for generation of minimal artifacts during analysis

^cArtifact of 7-ketocholesterol, values were corrected to account for 3,5-7-one initially present in the 7-ketocholesterol standard