Table 2.
ITC analysis of peptide interactions with hSPSB proteins
| Protein:Peptide | Kda (nM) | N (stoichiometric ratio) | ΔHbinding (kcal/mol) | TΔSbinding (kcal/mol) |
|---|---|---|---|---|
| hSPSB1:hPar-4b | 150 ± 12 | 0.94 | − 13.2 ± 0.1 | − 4.1 ± 0.1 |
| hSPSB1:VASAc | 40 ± 5 | 0.96 | − 20.5 ± 0.2 | − 10.6 ± 0.2 |
| hSPSB2:hPar-4 | 1520 ± 50 | 1.18 | − 16.2 ± 0.1 | − 8.4 ± 0.1 |
| hSPSB2:VASA | 147 ± 7 | 0.96 | − 23.9 ± 0.1 | − 14.7 ± 0.1 |
| hSPSB4:hPar-4 | 65 ± 4 | 1.19 | − 17.0 ± 0.1 | − 7.4 ± 0.1 |
| hSPSB4:VASA | 4.0 ± 0.7 | 0.97 | − 25.2 ± 0.1 | − 13.9 ± 0.1 |
a
Deviations represent the errors of the curve fit to the data obtained using the single binding site model used within the Origin software provided with the instrument.
b
hPar-4(67–81) has sequence NELNNNLPGGAPAAP.
c
VASA(184–203) has sequence DINNNNNIVEDVERKREFYI.