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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 30;1803(10):1198–1205. doi: 10.1016/j.bbamcr.2010.06.007

Figure 3.

Figure 3

Coimmunoprecipitation of c-Abl with Gal-3. MDA-MB-435 cell lysates were immunoprecipitated using anti-galectin-3 (lanes 1,4,5), anti-TCF-4 (lane 2) and anti-β-catenin (lane 3) antibody and immunoblotted with anti-c-Abl, anti-TCF-4, anti-β-catenin and anti-galectin-3 polyclonal antibodies. Some cells were treated with sucrose and lactose (line 4,5 respectively). To confirm equal loading of immunoprecipitated proteins 15% of IP mixture was run on 10% SDS-PAGE and visualized with Coomassie blue stain.