Figure 5.

CB8-2 binds a conformational epitope on TSG101. MALDI-TOF analysis were undertaken to determine the binding site of CB8-2 on TSG101. Panel A shows mass spectrometry analysis of MALDI-TOF using protease digested TSG101 incubated with CB8-2 monoclonal antibodies. Note the absence of three peaks which correspond to the three segments of TSG101 shown in panel B. Panel B illustrates the amino acid sequence of human TSG101 with the three regions recognized by the antibody highlighted in yellow.