Table 3.
Morphometry of cardiomyocytes isolated from NTG, TG and KO mice at 10 weeks after TAC or sham surgery
| NTG Sham | NTG TAC | TG Sham | TG TAC | KO Sham | KO TAC | |
|---|---|---|---|---|---|---|
| n | 8 | 8 | 6 | 9 | 7 | 6 |
| Cell Volume (×103 μm3) | 35.5±1.8 | 57.8±5.2* | 33.0±1.6 | 58.5±4.2* | 47.9±2.2# | 65.2±3.0* |
| CSA (μm2) | 259±11 | 351±30* | 242±14 | 359±17* | 309±13# | 388±25* |
| Cell Length (μm) | 137.2±4.2 | 164.7±2.8* | 136.4±2.7 | 163.2±7.0* | 155.0±5.4# | 168.2±4.3* |
| Cell Profile Area (μm2) | 3963±242 | 6157±311* | 3707±172 | 6027±463* | 5087±363# | 6778±496* |
LV cardiomyocytes isolated by collagenase perfusion were fixed in isotonic glutaraldehyde. A Coulter Channelyzer was used to determine the cell volume and microscopic image analyses were used to measure cell dimensions. Mean±SD.
*
P<0.05 compared to the sham of the same genotype;
#
P<0.05 compared to NTG sham.