Table I. Summary of cytochemical staining and immunolocalization of cell wall polymers in in vitro-grown Arabidopsis pollen tubes.
Data are based on observations at the fluorescence microscopy (FM) and electron microscopy (EM) levels. −, No labeling detected (at over 500-ms exposure time); ±, weak labeling detected; +, strong labeling detected. iw, Inner wall layer; na, not applicable; nd, not determined; ow, outer wall layer.
| Target | Probe | Back from the Tip |
Tip |
|
| FM Level | EM Level | FM Level | ||
| β-Glucan | Calcofluor white | + | na | + |
| Callose | Aniline blue | + | na | − |
| Anti-callose | nd | iw | nd | |
| Extensin | LM1 | − | nd | − |
| AGP | LM2 | ± | nd | + |
| MAC207 | ± | nd | + | |
| JIM13 | − | nd | − | |
| HG | JIM5 | + | nd | ± |
| JIM7 | ± | nd | + | |
| Galactan | LM5 | ± | nd | ± |
| Arabinan | LM6 | + | ow | + |
| LM13 | + | nd | + | |
| Xylogalacturonan | LM8 | + | nd | + |
| XyG | CCRC-M1 | + | iw/ow | + |
| LM15 | + | iw/ow | + | |
| Controls | − | − | − | |