Table III. Relative quantification of XyG oligosaccharides obtained after endo-glucanase digestion of Arabidopsis pollen tube wall.
Massa | Compositionb | Structurec | Relative Abundanced |
1,085 | Hex4Pent3 | XXXG | 14.7 ± 3.3 |
1,247 | Hex5Pent3 | XLXG/XXLG | 5.7 ± 0.6 |
1,289 | Hex5Pent3OAc1 | XLXG/XXLG + 1 OAc | 27.6 ± 3.9 |
1,393 | Hex5Pent3Dox1 | XXFG | 8.4 ± 0.5 |
1,435 | Hex5Pent3Dox1OAc1 | XXFG + 1 OAc | 41.2 ± 6.6 |
1,555 | Hex6Pent3Dox1 | XLFG | 2.5 ± 0.1e |
Mass of the fragments [M+Na+]+.
Hex, Hexose; Pent, pentose; OAc, O-acetyl substituent; Dox, deoxyhexose.
Based on XyG oligosaccharide structures found in Pauly et al. (2001).
Values are expressed as percentage and are means ± sd from MALDI spectra obtained after endo-glucanase digestion from three different Arabidopsis pollen tube cell wall extracts.
Relative abundance of this oligosaccharide corresponds to the total of the K+ and Na+ adduct fragments.