Figure 8.

AAMT activity correlates with transcript accumulation of aamt genes after different plant treatments. Plants were treated with jasmonic acid (JA), salicylic acid (SA), or S. littoralis larvae (Spod). Leaf material was harvested and split into two parts for different analyses. A, AAMT activity. Plant crude protein extracts were incubated with the methyl group donor [¹⁴C]SAM and anthranilic acid. The formed methyl ester was extracted from the assays and quantified using a scintillation counter. Means and se are shown (n = 4). B, Transcript accumulation of aamt genes. RNA isolated from leaf material was hybridized with a probe specific for maize aamt genes. The bottom panel shows the total RNA of the ethidium bromide-stained RNA gel. ctr, Control plants without treatment.