Complementation analysis of snc4-1D. A, Morphology of wild-type (WT), snc4-1D, WT::SNC4 (wild type transformed with a SNC4 genomic clone), and WT::snc4-1D (wild type transformed with a snc4-1D genomic clone) transgenic lines. All plants were grown on soil and photographed when they were 3 weeks old. B to D, Expression of PR1 (B), PR2 (C), and PDF1.2 (D) in wild-type, snc4-1D, WT::SNC4, and WT::snc4-1D transgenic lines. Values were normalized to the expression of ACTIN1. Error bars represent sd from averages of three measurements. E, Growth of H. a. Noco2 on wild-type, snc4-1D, WT::SNC4, and WT::snc4-1D transgenic lines. Infection was performed and scored as in Figure 1B. The values presented are averages of three replicates ± sd. [See online article for color version of this figure.]