Figure 7.

Restoration of ABA-, H₂O₂-, or NO-induced inhibition of blue light-dependent stomatal opening and H⁺ pumping by 1-BuOH. A, Epidermal peels were preincubated for 2 h in darkness and then illuminated with red light (RL; 150 μmol m^–2 s^–1) with or without blue light (BL; 10 μmol m^–2 s^–1) for 2 h. ABA (10 μm), H₂O₂ (1 mm), or SNP (100 μm) was added 30 min before and 1-BuOH (0.2%, v/v) was added 1 h before the light illumination. Results are means ± sd (n = 90, pooled from triplicate experiments). B, Guard cell protoplasts were preincubated for 40 min under red light (600 μmol m^–2 s^–1) and then illuminated with a pulse of blue light (100 μmol m^–2 s^–1, 30 s). ABA (0.1 μm), H₂O₂ (1 mm), or SNP (100 μm) was added 30 min before and 1-BuOH (0.2%, v/v) was added 40 min before the blue light pulse. Values shown are means ± se (n = 3). Asterisks indicate significant differences between treatments with and without 1-BuOH (P < 0.01).