Table III. Metabolites significantly regulated in root hairs in response to B. japonicum.
The predicted compound origin, the P value (pVal; comparison of inoculated versus uninoculated condition), and the fold change ratio (FC; inoculated/uninoculated) for each time point (t0, t12, t18, t24, t36, and t48 hours after inoculation with the symbiotic bacterium) are indicated. Metabolite abundance was also statistically analyzed by combining all the time points and comparing the metabolite abundance in all inoculated conditions versus all uninoculated conditions (pVal Comb). Significant changes are indicated in boldface.
| Compound | Metabolite Origin | pVal t0 | FC t0 | pVal t12 | FC t12 | pVal t18 | FC t18 | pVal t24 | FC t24 | pVal t36 | FC t36 | pVal t48 | FC t48 | pVal Comb |
| (Iso)flavonoid | ||||||||||||||
| Amino-flavonoid | Plant | 5.2E-02 | 2.5 | 2.4E-02 | 4.0 | 4.4E-04 | 4.8 | 5.2E-05 | 3.6 | 1.5E-03 | 2.9 | 1.6E-04 | 3.7 | 2.0E-13 |
| Aureusidin | Plant | 9.3E-01 | 1.2 | 3.5E-01 | 2.8 | 7.1E-01 | 1.3 | 7.7E-01 | 1.5 | 6.4E-01 | 1.3 | 2.8E-03 | 0.5 | 9.1E-01 |
| Isoliquiritigenin | Plant | 8.9E-01 | 1.0 | 2.8E-01 | 1.1 | 3.2E-03 | 1.7 | 3.3E-03 | 1.6 | 1.4E-03 | 1.4 | 1.1E-02 | 1.4 | 1.2E-08 |
| Apigenin 7-glucoronide | Plant | 3.8E-01 | 2.6 | 9.1E-01 | 1.4 | 1.3E-02 | 1.4 | 5.3E-01 | 1.0 | 7.4E-01 | 1.2 | 1.9E-02 | 0.7 | 3.8E-01 |
| Dihydrokaempferol b | Plant | 2.2E-01 | 0.8 | 1.7E-01 | 2.3 | 3.1E-01 | 1.4 | 7.8E-01 | 1.1 | 3.3E-01 | 1.7 | 1.3E-01 | 1.9 | 7.9E-03 |
| Afrormosin | Plant | 3.5E-01 | 0.9 | 3.0E-01 | 1.3 | 1.2E-02 | 1.5 | 5.5E-01 | 1.3 | 7.7E-02 | 1.4 | 1.4E-01 | 1.4 | 5.5E-03 |
| Genkwanin a | Plant | 9.0E-01 | 1.2 | 7.8E-02 | 1.5 | 1.4E-03 | 2.9 | 5.9E-01 | 1.6 | 1.7E-01 | 1.4 | 1.5E-01 | 1.3 | 5.2E-04 |
| Prunetin a | Plant | 3.0E-01 | 1.9 | 8.4E-02 | 1.5 | 5.6E-04 | 2.0 | 5.0E-01 | 1.6 | 1.8E-01 | 1.3 | 1.7E-01 | 1.3 | 9.2E-04 |
| Liquiritigenin | Plant | 8.2E-01 | 1.0 | 1.6E-01 | 1.1 | 8.7E-03 | 1.3 | 2.9E-01 | 1.2 | 4.0E-01 | 1.1 | 1.7E-01 | 1.2 | 2.7E-03 |
| Liquiritin | Plant | 8.1E-01 | 1.0 | 1.4E-01 | 1.1 | 7.5E-03 | 1.3 | 2.8E-01 | 1.2 | 3.9E-01 | 1.1 | 1.8E-01 | 1.2 | 2.4E-03 |
| Sissotrin | Plant | 1.1E-01 | 0.9 | 4.8E-01 | 1.0 | 1.4E-01 | 1.2 | 1.5E-01 | 1.3 | 2.3E-01 | 1.2 | 1.9E-01 | 1.5 | 4.1E-03 |
| Isorhamnetin 7-α-d-glucosamine | Plant | 6.7E-01 | 1.3 | 5.5E-01 | 1.0 | 2.5E-01 | 1.6 | 7.2E-03 | 0.5 | 5.5E-01 | 1.6 | 2.3E-01 | 2.1 | 7.8E-01 |
| Apigenin | Plant | 7.7E-01 | 1.0 | 2.0E-07 | 5.0 | 4.1E-02 | 4.4 | 8.8E-01 | 1.1 | 9.2E-01 | 1.0 | 3.1E-01 | 0.9 | 1.8E-01 |
| Prunetin b | Plant | 4.4E-02 | 0.6 | 8.4E-01 | 1.1 | 5.9E-05 | 4.7 | 5.4E-01 | 1.5 | 5.4E-01 | 0.8 | 4.0E-01 | 1.9 | 3.2E-02 |
| Delphinidin 3-rhamnoside-5-glucoside | Plant | 5.6E-01 | 0.9 | 2.0E-01 | 0.7 | 2.0E-01 | 0.5 | 1.7E-02 | 0.3 | 2.6E-01 | 0.7 | 6.0E-01 | 1.2 | 6.5E-03 |
| Naringenin 4′-O-glucoside | Plant | 2.0E-01 | 9.6 | 1.5E-02 | 1.4 | 3.4E-02 | 1.6 | 4.1E-01 | 1.1 | 4.0E-02 | 1.4 | 6.9E-01 | 1.1 | 6.1E-02 |
| Fatty acid | ||||||||||||||
| Lignoceric acid | Plant | 2.8E-02 | 0.8 | 3.2E-01 | 1.1 | 8.7E-02 | 1.4 | 3.2E-01 | 1.1 | 3.8E-01 | 1.1 | 3.7E-03 | 1.3 | 1.1E-02 |
| α-Eleosteric acid | Plant | 3.7E-01 | 1.2 | 6.1E-01 | 1.0 | 5.6E-01 | 1.1 | 6.7E-01 | 1.1 | 8.3E-01 | 0.9 | 6.0E-03 | 1.5 | 4.5E-01 |
| α-Linolenic acid | Plant | 3.5E-01 | 1.1 | 8.0E-01 | 1.0 | 3.7E-01 | 1.2 | 4.0E-01 | 0.9 | 6.6E-01 | 0.9 | 8.4E-03 | 1.5 | 5.7E-01 |
| Octadecenoic fatty acid | Plant | 6.2E-02 | 15.1 | 3.2E-02 | 2.6 | 5.2E-04 | 4.3 | 1.1E-02 | 2.6 | 3.5E-03 | 3.2 | 8.5E-03 | 3.3 | 1.0E-10 |
| Lauric acid | Plant/bacteria | 2.5E-01 | 0.8 | 5.9E-02 | 0.8 | 7.2E-01 | 1.0 | 2.8E-01 | 0.9 | 1.8E-01 | 0.9 | 3.3E-01 | 0.9 | 6.7E-03 |
| Myristic acid | Plant/bacteria | 1.8E-01 | 0.9 | 1.8E-02 | 0.9 | 3.5E-01 | 0.9 | 4.2E-01 | 0.9 | 2.2E-01 | 0.9 | 4.0E-01 | 0.9 | 6.2E-03 |
| Amino acid | ||||||||||||||
| l-Aspartic acid, N, O, O-TMS | Plant/bacteria | 7.1E-02 | 15.5 | 2.9E-05 | 5.0 | 6.4E-02 | 15.4 | 1.6E-03 | 7.4 | 3.8E-03 | 7.0 | 2.3E-03 | 6.4 | 1.0E-07 |
| l-Aspartic acid, O, O-TMS | Plant/bacteria | 7.2E-02 | 3.6 | 6.8E-05 | 5.4 | 5.9E-02 | 7.3 | 1.3E-02 | 9.7 | 1.7E-05 | 6.3 | 2.2E-02 | 7.9 | 5.7E-09 |
| l-Aspartic acid, 4TMS | Plant/bacteria | 4.3E-01 | 1.3 | 1.7E-01 | 1.6 | 7.8E-02 | 2.8 | 2.0E-02 | 3.5 | 9.9E-04 | 2.3 | 6.9E-02 | 3.6 | 8.2E-05 |
| 4-Aminobutyric acid, N, O, O-TMS | Plant | 1.3E-01 | 1.6 | 2.9E-01 | 0.8 | 7.6E-01 | 1.0 | 2.6E-03 | 0.7 | 2.9E-01 | 0.9 | 1.2E-01 | 0.8 | 2.1E-02 |
| l-Glutamic acid, O, O-TMS | Plant/bacteria | 1.3E-01 | 1.6 | 9.7E-01 | 1.2 | 6.8E-01 | 1.4 | 2.6E-03 | 0.7 | 5.5E-01 | 1.0 | 1.2E-01 | 0.8 | 1.2E-01 |
| Proline, N, O-TMS | Plant/bacteria | 7.8E-01 | 1.2 | 7.0E-01 | 1.0 | 1.5E-01 | 0.8 | 4.7E-01 | 1.1 | 4.8E-03 | 0.7 | 1.4E-01 | 0.8 | 1.0E-01 |
| Carboxylic acid | ||||||||||||||
| Lactic acid, O, O-TMS | Plant/bacteria | 6.6E-01 | 5.0 | 6.3E-02 | 70.1 | 5.0E-03 | 54.9 | 1.2E-02 | 19.0 | 1.2E-01 | 10.8 | 7.6E-03 | 64.9 | 3.2E-08 |
| Benzoic acid, O-TMS | Plant | 9.8E-01 | 1.1 | 2.4E-01 | 1.5 | 4.9E-01 | 1.2 | 7.7E-03 | 1.4 | 7.3E-01 | 1.0 | 2.4E-01 | 0.9 | 2.2E-01 |
| Sydnic acid | Plant | 1.4E-01 | 0.8 | 5.6E-01 | 1.3 | 4.1E-02 | 0.6 | 7.6E-01 | 2.0 | 3.4E-02 | 2.4 | 5.5E-01 | 1.3 | 6.6E-01 |
| Threonic acid, O, O, O, O-TMS | Plant | 2.4E-01 | 1.7 | 2.3E-02 | 1.6 | 2.5E-02 | 1.6 | 6.0E-01 | 1.1 | 7.7E-01 | 1.2 | 9.4E-01 | 1.0 | 1.6E-01 |
| Disaccharide | ||||||||||||||
| Trehalose, 8TMS | Plant/bacteria | 1.2E-01 | 1.4 | 5.2E-05 | 15.6 | 4.9E-05 | 17.6 | 3.8E-03 | 7.8 | 3.0E-03 | 32.7 | 9.9E-04 | 27.4 | 6.1E-11 |
| Maltose monohydrate MEOX1, TMS | Plant | 5.7E-03 | 1.8 | 2.1E-01 | 2.9 | 1.1E-01 | 7.0 | 4.6E-01 | 1.7 | 2.8E-03 | 32.6 | 4.9E-02 | 19.3 | 1.9E-04 |
| Maltose methoxyamine | Plant | 2.1E-03 | 1.6 | 3.6E-03 | 1.6 | 6.2E-03 | 1.8 | 6.0E-01 | 1.0 | 9.4E-03 | 1.8 | 2.3E-01 | 1.3 | 4.2E-03 |
| Glucosinolate | ||||||||||||||
| 4-Hydroyxindol-3-yl-methyl-glucosinolate | Plant | 3.2E-01 | 1.8 | 2.3E-02 | 0.6 | 7.9E-01 | 1.2 | 2.2E-01 | 2.5 | 6.0E-01 | 1.1 | 3.0E-02 | 2.1 | 2.7E-01 |
| 7-Methylsulfinyl-n-heptyl-glucosinolate | Plant | 7.7E-01 | 1.6 | 7.7E-01 | 1.3 | 9.1E-03 | 5.5 | 2.0E-02 | 0.5 | 8.4E-01 | 1.2 | 9.1E-02 | 0.6 | 4.4E-01 |
| Other | ||||||||||||||
| Beiwutine | Plant | 6.1E-01 | 0.9 | 1.0E-01 | 0.6 | 6.1E-01 | 1.4 | 1.9E-01 | 0.7 | 1.8E-01 | 0.8 | 2.1E-04 | 2.7 | 2.3E-01 |
| O-Methyl inositol, 5TMS | Plant | 6.0E-02 | 0.6 | 9.2E-01 | 4.8 | 5.8E-03 | 0.2 | 8.9E-01 | 6.0 | 2.6E-01 | 0.9 | 2.0E-01 | 0.4 | 3.0E-02 |
| Ascochalasin | Plant | 8.4E-01 | 1.0 | 3.8E-01 | 0.7 | 9.1E-01 | 1.2 | 3.6E-02 | 1.6 | 6.0E-01 | 1.1 | 2.8E-02 | 0.7 | 7.5E-01 |
| 3,3′,4,4′5-Pentahydroxystilbene | Plant | 7.5E-01 | 1.4 | 8.8E-01 | 1.1 | 3.9E-04 | 2.4 | 4.8E-01 | 1.4 | 4.2E-01 | 1.3 | 2.9E-01 | 1.6 | 9.7E-03 |
| 4’,5′-Trihydroxy-3′,6′′,6′′-trimetylpyranol (2′′,3′′:6′′,5′′)dihydrochalcone | Plant | 1.2E-01 | 0.8 | 1.7E-01 | 3.0 | 6.2E-03 | 2.3 | 2.7E-01 | 0.8 | 3.5E-01 | 0.8 | 7.8E-01 | 0.9 | 8.9E-01 |
| Neoxanthin | Plant | 4.7E-01 | 1.3 | 5.2E-01 | 2.3 | 3.8E-01 | 0.8 | 1.7E-01 | 0.6 | 3.2E-03 | 0.6 | 1.1E-01 | 0.6 | 1.5E-02 |
| Medicagenic acid 3-O-triglucoside | Plant | 4.4E-01 | 2.6 | 1.2E-01 | 7.2 | 4.1E-01 | 1.8 | 3.6E-01 | 0.8 | 8.1E-03 | 2.3 | 4.9E-01 | 1.5 | 3.6E-02 |
| α-Glycerophosphate ester, TMS | Plant | 8.1E-01 | 1.2 | 2.4E-02 | 1.7 | 3.0E-01 | 1.6 | 5.4E-01 | 1.7 | 3.7E-02 | 3.1 | 5.9E-01 | 1.2 | 1.9E-02 |
| Cytosine, N, N-TMS | Plant/bacteria | 2.2E-01 | 0.6 | 2.2E-01 | 0.8 | 1.6E-02 | 0.5 | 3.3E-01 | 1.0 | 9.0E-02 | 0.7 | 5.8E-01 | 0.9 | 2.5E-03 |
| Xylose methoxyamine | Plant/bacteria | 4.5E-02 | 2.0 | 4.4E-01 | 0.9 | 3.4E-01 | 1.2 | 5.4E-01 | 1.0 | 6.7E-01 | 1.0 | 8.6E-03 | 0.8 | 3.3E-01 |
| Rha-Hex-Hex-Hex-gypsogenic acid | Plant | 2.5E-01 | 1.3 | 2.7E-01 | 1.1 | 7.0E-03 | 1.3 | 9.4E-01 | 1.0 | 1.6E-02 | 1.3 | 2.1E-01 | 1.1 | 5.9E-02 |