Table I. Survival, relative conductivity, and Fv/Fm in mutant plants (mvp-2/mvp-2= m/m) and plants carrying at least one wild-type VRN-1 allele (Mvp-2/− = M/−).
Sixty-day-old plants were acclimated at 10°C for 19 d, 4°C for 12 d, and –2°C for 12 h before the freezing treatment. P values correspond to factorial ANOVAs including temperature, genotype, and their interactions in the model. Experimental conditions are described in “Materials and Methods.”
| Parameter | Freezing Temperature |
Factorial ANOVA (P between Genotypes) | |||
| −9°C |
−11°C |
||||
| M/− | m/m | M/− | m/m | ||
| Fv/Fm | 0.56 | 0.78 | 0.56 | 0.81 | <0.0001 |
| Relative conductivity (%) | 12.0 | 10.1 | 17.3 | 11.9 | 0.05 |
| No. of plants that regrew per total (%) | 0/50 (0%) | 26/30 (87%) | 0/59 (0%) | 11/24 (46%) | NAa |
a
All Mvp-2/− plants failed to regrow; therefore, there is no variance within this class to perform an ANOVA. The differences between mvp-2/mvp-2 and Mvp-2/− were obvious.