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. 2010 Jun 18;153(4):1577–1586. doi: 10.1104/pp.110.157461

Figure 1.

Figure 1.

Peroxisomal mutants are defective in IBA-to-IAA conversion. A, Auxin response of Col-0, tir1-1, axr2-1, pex6-1, pxa1-1, acx1-2 acx2-1, chy1-10, and ibr1-2 ibr3-1 ibr10-1. Mean primary root lengths (+se; n ≥ 12) of 8-d-old seedlings grown under yellow-filtered light at 22°C on medium supplemented with ethanol (mock), 100 nm IAA, or 10 μm IBA are shown. B, Suc dependence of Col-0, tir1-1, axr2-1, pex6-1, pxa1-1, acx1-2 acx2-1, chy1-10, and ibr1-2 ibr3-1 ibr10-1. Mean hypocotyl lengths (+se; n = 12) of seedlings grown in the absence or presence of 0.5% Suc were measured 4 d after transfer of 1-d-old seedlings to the dark. C, In planta conversion of IBA to IAA. Eight-day-old Col-0, tir1-1, axr2-1, pex6-1, pxa1-1, acx1-2 acx2-1, chy1-10, and ibr1-2 ibr3-1 ibr10-1 light-grown seedlings were incubated for 1 h in the presence of 10 μm [13C8-15N1]IBA and the resultant [13C8-15N1]IAA levels were measured. Wt, Col-0.